bio rad bio plex protm ii wash station Search Results


custom ordered bio plex protm rat cytokine assay kit  (Bio-Rad)
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Bio-Rad custom ordered bio plex protm rat cytokine assay kit
Custom Ordered Bio Plex Protm Rat Cytokine Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse bio plex protm mouse cytokine 23 plex assay  (Bio-Rad)
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Mouse Bio Plex Protm Mouse Cytokine 23 Plex Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio plex protm assays  (Bio-Rad)
99
Bio-Rad bio plex protm assays
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cyclin d1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology cyclin d1
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
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p6hrt prot vector  (Bio-Rad)
88
Bio-Rad p6hrt prot vector
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
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bio plex protm human assay  (Bio-Rad)
96
Bio-Rad bio plex protm human assay
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
Bio Plex Protm Human Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio plex protm ii wash station  (Bio-Rad)
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Bio-Rad bio plex protm ii wash station
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
Bio Plex Protm Ii Wash Station, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blot loading tip  (Bio-Rad)
94
Bio-Rad western blot loading tip
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
Western Blot Loading Tip, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio plex protm mouse cytokine 23 plex assay  (Bio-Rad)
93
Bio-Rad bio plex protm mouse cytokine 23 plex assay
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
Bio Plex Protm Mouse Cytokine 23 Plex Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egf receptor  (Bio-Rad)
93
Bio-Rad egf receptor
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
Egf Receptor, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp mdm2 hs00242813 m1  (Thermo Fisher)
90
Thermo Fisher gene exp mdm2 hs00242813 m1
FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of <t>cyclin</t> <t>D1</t> and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.
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Image Search Results


FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of cyclin D1 and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.

Journal: Journal of Biological Chemistry

Article Title: Farnesoid X Receptor, through the Binding with Steroidogenic Factor 1-responsive Element, Inhibits Aromatase Expression in Tumor Leydig Cells

doi: 10.1074/jbc.m109.052670

Figure Lengend Snippet: FIGURE 8. CDCA reverses the effects of AD on R2C cell proliferation. A, R2C cells were transfected with control siRNA or FXR siRNA for 24 h and then transiently transfected with the XETL promoter plasmid. Cells were treated with 50 M CDCA in the with or without 100 nM AD for 24 h. These results represent the mean S.D. of three different experiments. In each experiment, the activities of the transfected plasmids were assayed in triplicate transfections. *, p 0.01 with respect to the vehicle. **, p 0.01 CDCA AD treated versus AD alone. B, R2C cells were treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treatedversusADalone.C,R2Ccellswereseeded(10,000/well)in0.5%agaroseandtreatedasdescribedabove. Cells were allowed to grow for 14 days and then the number of colonies 50 m were quantified and the results graphed. The results represent the mean S.D. of three different experiments each performed with triplicate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. D, total proteins extracted from R2C cells treated with vehicle (), 100 nM AD, 50 M CDCA, and AD CDCA for 24 h were used for immunoblot analysis of cyclin D1 and cyclin E. -Actin was used as a loading control. The histograms represent the mean S.D. of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as percentages of the control, which was assumed to be 100%. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone. E, aromatase protein in R2C cells that were not transfected () or transfected with siRNA targeted rat aromatase mRNA sequence as described under “Experimental Procedures” for 24, 48, and 72 h. GAPDH was used as loading control. F, R2C cells were transfected with control siRNA or Arom siRNA for 48 h and then treated with 100 nM AD in the presence or not of 50 M CDCA for 24 h. Thymidine incorporation assay was performed. The results represent the mean S.D. of three different experiments each performed with tripli- cate samples. *, p 0.01 AD treated compared with vehicle. **, p 0.01 CDCA AD treated versus AD alone.

Article Snippet: Antibodies against FXR, -actin, GAPDH, cyclin D1, cyclin E, and lamin B were from Santa Cruz Biotechnology (Santa Cruz, CA), antibody against Aromatase from Serotec (Raleigh, NC), antibody against SF-1 was kindly provided from Dr. K. Morohashi (National Institute Basic Biology, Myodaiji-cho, Okazaki, Japan), and anti-LRH-1 antibody was kindly provided by Dr. Luc Belanger (Laval University, Quebec, Canada).

Techniques: Transfection, Control, Plasmid Preparation, Thymidine Incorporation Assay, Western Blot, Sequencing